A Polyamine Oxidizing Enzyme as a Drug to Treat Breast Cancer
Abstract
The research is aimed at testing two polyethylene glycolated (PEGylated) forms of bovine serum amine oxidase (SAO) as effective treatments for breast cancer using a mouse model. Hopefully, this approach, or a variation thereof, can be used as a new a therapy for breast and other cancers in humans. Currently, a large quantity of pure bovine PAO is available, which was obtained from 10 gallons of fresh cow blood. A final purification step has been used to produce large quantities of extremely pure SAO. Attempts to find a cost-effective, practical method for the degycosylation of SAO were unsuccessful. Using several different reagents, we have found two that will be used to PEGylate SAO. Before testing as an anti-tumor agents, both PEGylated SAO derivatives will be tested for toxicity by using nontumorigenic mice. Once it is established that these agents do not have significant side effects, they will be tested for the ability to slow the growth or shrink the size of breast tumors implanted in test mice. PEGylated SAO should target tumors but have little effect on normal tissue. Once concentrated extracellularly in a tumor, active PEGylated SAO will oxidize acetylated polyamines, which are excreted by tumor cells in large quantities. When the acetylated polyamines are oxidized, cytotoxins are generated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2007
- Accession Number
- ADA484624
Entities
People
- William S. Mcintire
Organizations
- Northern California Institute for Research and Education