An Epigenetic Link to Prostate Cancer

Abstract

The aim of this proposal is to investigate the role of Ezh2 in prostate cancer progression. Ezh2 is a histone methyl transferase whose activity requires to be part of a multi-protein complex. We have hypothesized that the complex composition would change during the prostate tumorigenic progression. To address this question, we have generated a knock-in mouse where Ezh2 is tagged to allow the immunopurification of its associated polypeptides from limited amount of starting material (mouse prostate). The knock-in mice are being generated. Using the targeted ES cells, I am currently setting up the micropurification protocol. One of the Ezh2 associated protein is Eed, different isoforms of this protein are detected in vivo. These isoforms are supposed to be key determinants of Ezh2 substrate specificity. I have characterized these isoforms and generated tools allowing the overexpression of each isoforms independently.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2006
Accession Number
ADA484689

Entities

People

  • Raphael F. Margueron

Organizations

  • University of Medicine and Dentistry of New Jersey

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Department Of Defense
  • Enzymes
  • Gene Expression
  • Information Operations
  • Mass Spectrometry
  • Materials
  • Neoplasms
  • New Jersey
  • New York
  • Prostate
  • Prostate Cancer
  • Proteins
  • Substrate Specificity
  • Tissues
  • Two Dimensional
  • Universities

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics