Prostate Cancer Gene Discovery Using ROMA
Abstract
We hypothesized that a subset of men who develop prostate cancer (PCa) do so as a result of an inherited chromosomal deletion or amplification affecting the function of one or more critical prostate cancer susceptibility genes. These chromosomal abnormalities and the affected gene(s) can be identified using the novel technique ROMA. Together with our collaborators at Cold Spring Harbor we performed ROMA analysis of 90 PCa patients each with a strong family history of PCa (having at least two affected first degree relatives). The majority of these men had either early onset PCa (diagnosis before age 60) or evidence of advanced disease (non-organ confined disease). Over 1100 copy number polymorphism (CNPs) counting many recurring CNPs were observed in these 90 patients although we were not able to verify many of these CNPs using quantitative PCR. Additionally we were able to rule out a causal role for a number of CNPs based on lack of cosegregation with disease in families in which multiple affected members have been subjected to ROMA analysis. From assaying a CNP on chromosome 2 in a large population of PCa cases and contrnls we were able to show a significant association of this CNP with risk of PCa diagnosis. Currently we are examining other common and rare CNPs for evidence of association with PCa risk.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2007
- Accession Number
- ADA484704
Entities
People
- William B Isaacs
Organizations
- Johns Hopkins University