Cadmium, Zinc, and Selenium Levels in Carcinoma of the Human Prostate
Abstract
The purpose of this study is to examine associations of zinc (Zn), selenium (Se) and cadmium (Cd), and development of prostate cancer. The objectives are: 1) to establish reliability of using formalin-fixed paraffin-embedded (FFPE) prostate tissue for analysis of Zn, Se and Cd tissue by comparing their levels in the fresh specimen; 2) to enhance the knowledge of prostate cancer environmental etiology by examining the association of Cd, Zn, Se with the development and progression of cancer on the basis of using FFPE prostate tissue specimen; 3) to enhance the use of the AFIP National Tissue Repository of archival tissues. Based on use of high-resolution inductively-coupled mass-spectrometry (ICP-MS), we developed a method for analysis of Zn, Cd, Se and also iron (Fe) in FFPE prostate. Our results demonstrated that in comparison with fresh tissue, in FFPE tissue the recoveries of Se, Fe, Cd and Zn were progressively decreased as 97+/-11%, 82+/-22%, 59+/-23% and 24+/-11%, respectively. Thus, the use of correction factors, determined as k=0.16 for Se, k=0.20 for Fe, k=0.27 for Cd and k=0.67 for Zn, is required to estimate the retrospective levels of these elements in the parental non-processed fresh (wet) prostate tissue. The developed methodology was applied to analyze the levels of Zn, Cd, Se and Fe in archival FFPE tissue specimens to study correlation between the metals content and prostate cancer development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2008
- Accession Number
- ADA484709
Entities
People
- Andrey Sarafanov
- Jose A. Centeno
Organizations
- Armed Forces Institute of Pathology