The Role of YYI in Prostate Cancer

Abstract

We have successfully generated Lentiviruses vector carrying yy1 siRNA (to knock down YY1) or YY1 overexpression cassette. In the cell culture studies the siRNA YY1 could efficiently knockdown the endogenous YY1 of Pten+/- mouse prostate epithelial cells (MPECs) while the YY1 cDNA could be overexpressed in Pten+/+ MPECs. Before conducting in vivo studies we wanted to test the effect of YY1 expression to the cell proliferation in vitro using the three dimensional (3-D) collagen culture system which mimics the in vivo prostate cell growth environment. We observed that the overexpression of YY1 enhanced the cell growth and formation of branching structure of Pten+/+ cells in the 3-D culture system. We used these cells in the renal grafting experiments to study the effect of YY1 expression to the prostate cancer formation in vivo. The renal grafts have been collected and further studies are in the process.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2008
Accession Number
ADA484715

Entities

People

  • Guangchao Sui

Organizations

  • Wake Forest University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Cassettes
  • Cell Line
  • Cells
  • Collagen
  • Culture Techniques
  • Epithelial Cells
  • Gene Expression
  • Infection
  • Knocking
  • Neoplasms
  • Prostate
  • Prostate Cancer
  • Proteins
  • Stem Cells
  • Three Dimensional
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Microwave Engineering.
  • Oncology (Cancer Research).