Targeted Elimination of PCDH-PC Expressing Prostate Cancer Cells for Control of Hormone-Resistant Prostate Cancer
Abstract
Protocadherin-PC (PCDH-PC) is a male-specific gene that is highly upregulated in androgen sensitive prostate cancer cells by androgen deprivation. Based on our preliminary work, we proposed that PCDH-PC protects prostate cancer cells from cell death subsequent to hormone therapy. Work in the project seeks to develop shRNA and antisense oligonucleotide agents that block PCDH-PC expression and to test whether suppression of PCDH-PC expression increases prostate cancer cell death under androgen-free conditions and whether this action blocks the development of hormone-resistant prostate cancers in mice xenografted with human prostate cancer cells. During the previous period we successfully developed shRNA and antisense oligonucleotide agents that target and suppress PCDH-PC expression. These agents are surprisingly toxic for cultured androgen-sensitive prostate cancer cells even when androgens are present and the results suggest that low level expression of PCDH-PC might contribute to prostate cancer cell survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2007
- Accession Number
- ADA484766
Entities
People
- Ralph Buttyan