A Rat Model for Human Ductal Carcinoma In Situ
Abstract
Our objective is to develop a rodent model for human DCIS and LCIS in which lesions of diverse phenotypes can be induced and characterized as to their malignant potential that could be characterized in a linear manner. The majority of mammary cancers induced in rats by carcinogen treatment are ovarian hormone dependent for their growth. We tested whether treatment of ovariectomized rats with growth factors during carcinogen treatment would result in a higher percentage of hormone independent mammary cancers. Ovariectomized rats were treated with 10 ug per day of either EGF or IGF-1. On the fourth day of treatment the rats were treated with the carcinogen N-methyl-N-nitrosourea. The rats were treated for an additional 3 days with growth factor and they were then treated with either estradiol prngesterone or estradiol plus progesterone to promote mammary carcinogenesis. The resultant mammary cancers were tested for the expression of estrogen receptor and progesterone receptor to determine their hormone dependent or independent phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2005
- Accession Number
- ADA484809
Entities
People
- Satyabrata Nandi
Organizations
- University of California, Berkeley