Identification and Characterization of Genomic Amplifications in Ovarian Serous Carcinoma
Abstract
The purpose of this proposed study is to apply genome-wide technologies to analyze ovarian cancer genome and transcriptome. In the past year, we have made substantial progress toward identifying the DNA copy number changes at a genome-wide scale and studying the molecular mechanisms of the most promising cancer-associated genes within new amplicons. Specifically, we found that SNP array analysis can provide a similar performance to profile DNA copy number alterations as digital karyotyping because of recent advances in technology refinement. Furthermore, SNP arrays would allow us to analyze more samples because of its lower cost. As a result, we have analyzed more than 40 affinity purified ovarian serous tumors and our results demonstrated that CCNE1, Notch3, Rsf-1, AKT2 and PIK3CA are among the most frequently amplified loci in high-grade serous carcinomas. Novel amplifications and sub-chromosomal deletions were also detected by the SNP array analysis. Our study is the first comprehensive analysis of the detailed amplicon profiles in purified ovarian serous tumors. In addition, we have further characterized the biological functions of the two of the commonly amplified genes, Notch3 and Rsf-1, in ovarian carcinoma. The results from this study shall provide new insight into how amplified genes contribute to survival and growth of cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2008
- Accession Number
- ADA485356
Entities
People
- Tian-li Wang
Organizations
- Johns Hopkins University