Contrast Agents for Micro-Computed Tomography of Microdamage in Bone

Abstract

Novel methods have been developed for detecting damaged bone tissue using micro-computed tomography (micro-CT) and contrast agents with higher x-ray attenuation than bone. The ability to detect the presence and to a limited extent the morphology of microdamage in cortical and trabecular bone using micro-CT was demonstrated using a barium sulfate (BaSO4) stain. Gold nanoparticles (Au NPs) were prepared and functionalized with L-glutamic acid for chelating calcium in damaged bone tissue. The functionalized Au NP contrast agent exhibited damage-specificity confirmed by optical electron and AFM imaging. Functionalized Au NPs were also conjugated with fluorescein to produce a dual contrast agent suitable for both fluorescent microscopy and micro-CT. The small size ( 40 nm) and colloidal stability of functionalized Au NP solutions enabled the contrast agent to readily diffuse into bone tissue which suggests that the contrast agent is deliverable.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2008
Accession Number
ADA485704

Entities

People

  • Ryan K. Roeder

Organizations

  • University of Notre Dame

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Communities of Interest

  • Biomedical

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  • Alcohols
  • Chemical Synthesis
  • Chemistry
  • Electron Microscopy
  • Health Services
  • Imaging Techniques
  • Materials
  • Materials Science
  • Mechanical Engineering
  • Mechanics
  • Medical Personnel
  • Metallic Nanoparticles
  • Microscopy
  • Modulus Of Elasticity
  • Tomography
  • X Rays
  • X-Ray Computed Tomography

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  • Analytical Chemistry
  • Immunology and Pathology
  • Medical Imaging.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech
  • Microelectronics