Keratinocyte Spray Technology for the Improved Healing of Cutaneous Sulfur Mustard Injuries
Abstract
The purpose of the current research is to determine whether the spray-on application of allogeneic keratinocytes in suspension will improve epidermal wound healing of vesicating burns induced by the chemical warfare agent sulfur mustard (HD). A beige SCID mouse model is used for these experiments which are being carried out in two phases. The first phase is dose ranging. The second phase tests the efficacy of spray keratinocytes (Universal Donor) at healing HD injuries. Dose ranging was carried out using HD (0, 80, 160, 320 mug) in methylene chloride delivered to the dorsum of depilated mice within an 8 mm diameter cloning ring. Under these conditions, the vehicle control caused patchy epidermal and follicular necrosis. As expected, the HD treatment generated confluent epidermal and follicular necrosis, endothelial cell necrosis, thrombi, and extravasation of fibrin and inflammatory infiltrate in the loose connective tissue. Induction of inflammation was independent of necrosis. Because of the confounding effects of methylene chloride, dose ranging and phase II experiments will be conducted with HD diluted in ethanol.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2008
- Accession Number
- ADA485720
Entities
People
- Marcia Simon
- Steve A. Mcclain
- Thomas Zimmerman
Organizations
- State University of New York