Disruption of the Circadian Rhythms of Gene Expression and the Development of Breast Cancer

Abstract

This project uses a mouse model to examine the effects of rhythm disruption on the expression of genes, the growth of breast cancer xenografts and spontaneous mammary tumours. Task 2: Task has been completed except for some additional gene expression assays. Task 3: Collection of tissues from the PyMT transgenic mice has been partially completed. Second cohort of animals are currently growing under the control and shiftwork simulation conditions. Task 4: A viable SCID+Clock 19 mouse colony has been established and expanded. These mice will be used in a xenograft experiment (Task 5). Task 6: We have commenced this Task and have demonstrated partial knock-down of Bmal1 expression. Experiments are continuing to clonally expand the cell lines and conduct functional assays on them.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2008
Accession Number
ADA486140

Entities

People

  • David J. Kennaway

Organizations

  • University of Adelaide

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Brain
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Circadian Rhythms
  • Data Analysis
  • Gene Expression
  • Metastasis
  • Neoplasms
  • Simulations
  • Statistical Analysis
  • Xenografts

Fields of Study

  • Biology

Readers

  • Circadian Sleep-Wake Regulation and Chronobiology
  • Clinical Trial Research.
  • Oncology (Cancer Research).