Modification of BRCA1 Breast Cancer Risk by Coffee Consumption: Potential Mechanisms for Biologic Effect
Abstract
The purpose of this study was to investigate the role of coffee and caffeine in the function of the DNA repair protein BRCA1 and to determine whether or not coffee and/or caffeine prevent BRCA1 hereditary breast cancer. We have bred the necessary genetically engineered mice for the animal study to determine whether coffee decaffeinated coffee or caffeine prevents BRCA1 hereditary breast cancer. We applied for a no cost extension to complete these studies during the next year so we will know whether or not caffeine prevents breast cancer in the animal model. We have also analyzed the 1853 mutation in the BRCA1 mutant cell line HCC1937 and shown that is unaffected by DNA damaging agents including caffeine although the full length wildtype BRCA1 protein is affected. We also show that the BRCA1 mutant proteins 1853 and Cys61Gly show a loss of nuclear localization and are defective in DNA repair and radiation response. We are preparing a manuscript to report this finding.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2007
- Accession Number
- ADA486173
Entities
People
- Jeffrey R Holt
Organizations
- University of Colorado Health