Identification of Tumor Suppressor Genes by Genetic and Epigenetic Genome-Scanning
Abstract
We used systematic multiplex rt-pcr and dna microarray hybridization methods to examine the expression of the genes in the chromosomal regions of 18q21-qter, 8p, and 1p33-pter, which are often decreased in copy number in breast tumors and cell lines derived from breast cancer. We identified dozens of genes in the chromosomal regions whose expression was frequently diminished or lost in breast cancer cell lines that were examined. We confirmed the results by real-time qrt-pcr. We also examined the expression of those genes in the clinical specimens of breast cancer and observed the down-regulation in expression of some of them in the clinical specimens of breast cancer. Those genes included ccbe1, tcf4, np_115536.1, and np_689683.2 in 18q21-qter, and myom2, np_859074, np_001034551, nrg1, phyip (phyhip), q7z2r7, sfrp1, and sox7 in 8p.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2008
- Accession Number
- ADA486546
Entities
People
- Fumiichiro Yamamoto
Organizations
- Sanford Burnham Prebys Medical Discovery Institute