Knockout AR in Prostate
Abstract
Prostate cancer may progress from an androgen-dependent to an androgen-independent state. The androgen receptor (AR) is expressed throughout this progression. We would like to understand the AR role in this progression. Using lox-Cre methodology, we have generated the mice in which AR function can be abolished in the entire animal (ARKO) or in a tissue specific manner. We will further generate mice that have AR knocked out in prostate only or in different stages to be used to study how prostate cancer progresses. Our Proposal aims follow. 1: Generate mice lacking a functional AR in prostate epithelium. 2: Generate inducible ARKO mouse line. These mice will be used to determine potential effect of androgen in absence of AR on prostate growth/maintenance. 3: Determine AR role in prostate cancer development/progression by crossing ARKO mice (from 1 and 2) with TRAMP mice. Comparing these mice will enable us to examine AR role in TRAMP induced prostate cancer and permit determination of points in prostate cancer requiring AR function. 4: Determine AR role in tumorigenicity of androgen-dependent and androgen-independent AR knockout prostate cancer cell lines. The effect of AR loss in these cells will be examined for ability to generate/promote tumors in mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2007
- Accession Number
- ADA486607
Entities
People
- Chawnshang Chang
Organizations
- University of Rochester