Developing a Zebrafish Model of NF1 for Structure-Function Analysis and Identification of Modifier Genes

Abstract

This progress report summarizes the first year of activity of this project focused on the identification and characterization of the zebrafish orthologs of the neurofibromatosis type 1 genes. This project involves work within the Epstein laboratory and collaboration with the laboratory of Dr. Thomas Look at the Dana Farber Cancer Institute as a sub-contract. This progress report summarized the collaborative work including results from both groups. During the first year, significant progress has been made in the isolation and characterization of two zebrafish orthologs, znf1a and znf1b. The genes have been sequenced and their expression patterns identified. The knockdown phenotypes have been characterized and include overgrowth of glia and cardiovascular abnormalities. Work towards identification of stable mutant fish lines has begun. Manuscripts are in preparation.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2008
Accession Number
ADA486682

Entities

People

  • Jonathan A. Epstein

Organizations

  • University of Pennsylvania

Tags

DTIC Thesaurus Topics

  • Abnormalities
  • Blood
  • Cardiovascular Abnormalities
  • Cardiovascular System
  • Cell Physiological Processes
  • Cells
  • Confocal Microscopy
  • Congenital Abnormalities
  • Diseases And Disorders
  • Fish
  • Genes
  • Genetic Structures
  • Genetics
  • Identification
  • Pericardial Effusion
  • Phenotypes
  • Spinal Cord

Readers

  • Molecular Genetics
  • Molecular and Cellular Biology
  • Technical Research and Report Writing.