Characterization of NF1 Protein Ubiquitination
Abstract
This project aimed at characterization of NF1 protein ubiquitination system by identifying ubiquitination sites and ligases for neurofibromin. We established the method for obtaining ubiquitinated neurofibromin for mass-spectrometric sequencing and identification of ubiquitination sites of neurofibromin. We confirmed neurofibromin ubiquitination and degradation after mitogenic stimulation by serum and LPA. Cbl-Ring finger ubiquitin ligase mediates ubiquitination and degradation of a variety of signaling proteins in the tyrosine kinase receptor signaling pathway. As one of efforts for identifying neurofibromin ubiquitin ligases, we discovered that ubiquitination and degradation of neurofibromin was induced by Cbl which may be responsible for neurofibromin ubiquitination upon activation of tyrosine-kinase receptors.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2008
- Accession Number
- ADA486692
Entities
People
- Koko Murakami
- Victor A. Fried
Organizations
- New York Medical College