Prion Transport to Secondary Lymphoreticular Tissues
Abstract
The long-term objective of this proposal is to identify mechanisms of prion transport to secondary lymphoreticular system (LRS) tissues. The hypothesis to be tested is that following peripheral exposure to prions; host proteins (e.g. complement) bind prions allowing for trapping by cells in the spleen and enhancing uptake by macrophages which are cells that are responsible for destruction of foreign proteins. To investigate this hypothesis we will examine the disease development of a prion strain (DY TME) that does not replicate in the spleen of hamsters. This system will provide details into the host factor(s) involved in transport of prions to cells in the LRS. We have shown the susceptibility of HY and DY TME to phagocytosis and degradation by a murine macrophage cell line. We have studied the effects of prion infection on phagocytic ability and cell viability. We have shown differences in the spatial and temporal spread of the HY and DY TME agents in LRS tissues following intraperitoneal inoculation. We have shown gender specific responses to intraperitoneal DY TME inoculation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2008
- Accession Number
- ADA487030
Entities
People
- Jason C. Bartz
Organizations
- Creighton University