Rapid Detection of Bacterial Antibiotic Resistance: Preliminary Evaluation of PCR Assays Targeting Tetracycline Resistance Genes

Abstract

The rapid spreading of bacterial antibiotic resistance and the lack of methods that would allow fast and accurate determination of the resistance profile of an infectious agent have become a major concern for public health. In the context of biological warfare or a bioterrorist attack, resistance against commonly used antibiotics would severely compromise the capabilities of the responsible agencies to protect large numbers of exposed individuals against a potentially lethal infection. In this study the possibility of developing polymerase chain reaction (PCR) assays for rapid detection of tetracycline resistance genes was evaluated. PCR assays were designed for seven resistance genes that frequently occur in Gram-positive and Gram-negative species and tested on eight tetracycline resistant clinical isolates. Positive results were obtained from seven of the eight species tested.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2007
Accession Number
ADA487261

Entities

People

  • Matthias R. Dorsch

Organizations

  • Defence Science and Technology Group

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Bacterial Infections
  • Bacteriology
  • Biological Warfare
  • Biological Warfare Agents
  • Chemical Synthesis
  • Chemistry
  • Chemotherapy
  • Gram-Negative Bacterial Infections
  • Infection
  • Microbiology
  • Pathogenic Bacteria
  • Polymerase Chain Reaction
  • Q Fever

Readers

  • Critical Infrastructure Protection in CBRN and WMD Threats.
  • Microbial Pathology
  • Systems Analysis and Design