Development of Micro-Scale Assays of Mammary Stem and Progenitor Cells

Abstract

This portion of the work proposed focused on employing and validating microtechnology for in vitro studies of primary mammary gland cell characteristics. Specific attention has been paid to developing more quantitative methods for analyzing microfluidic cell cultures using In Channel Westerns. Also, understanding how the microfluidic culture platform differs from traditional macro-scale techniques is critical and was explored in more depth than previously done. By thoroughly understanding how this culture platform affects the cellular baseline first, better and more efficient data collection can be performed, thus requiring fewer primary cells.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2008
Accession Number
ADA488004

Entities

People

  • Amy L. Paguirigan

Organizations

  • University of Wisconsin–Madison

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bioassay
  • Biomedical Engineering
  • Blood
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Culture Techniques
  • Dna Microarrays
  • Epithelial Cells
  • Fluid Flow
  • Growth Factors
  • Mammary Glands
  • Metabolism
  • Proteins
  • Stem Cells
  • Three Dimensional

Readers

  • Immunology and Pathology
  • Integrated Circuit Design and Technology.
  • Theoretical Analysis.