Establishment of an 'In Vitro Cell-Based System' to Assay Radiation Sensitivity in Breast Cancer

Abstract

The purpose of this proposal is to develop an 'in vitro cell-based system' to study radioresistance in sporadic breast cancer. By performing colony formation assays in response to X-ray treatment we have shown that there is a wide range of sensitivities to this form of treatment. Interestingly, we have also observed that p53 status does not accurately predict response to radiation treatment. Using correlation studies, we have identified novel predictors of IR resistance/sensitivity. We have sensitized two breast cancer cell lines to X-ray treatment by using RNAi-based technology to inhibit Artemis function. In the future, these observations may have a significant clinical impact on the way radiation oncologists treat breast cancer patients.

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Document Details

Document Type
Technical Report
Publication Date
May 31, 2008
Accession Number
ADA488058

Entities

People

  • Gregory Langland

Organizations

  • University of California, Berkeley

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Fatty Acids
  • Gene Expression
  • Lipid Metabolism
  • Medical Personnel
  • Neoplasms
  • Polymerase Chain Reaction
  • Radiation
  • Resistance
  • Sensitivity
  • X Rays

Fields of Study

  • Medicine
  • Physics

Readers

  • Molecular and genetic basis of cancer.
  • Nuclear and Radiation Engineering.
  • Oncology (Cancer Research).