A Role for Ubiquitin Binding in Bcr-Abl Transformation
Abstract
We have previously identified a docking site for ubiquitin in the amino-terminus of p210 BCR/ABL. Since ubiquitin binding has been implicated in both the intra- and inter-molecular regulation of many signaling molecules this association may have important clinical consequences for chronic myelogenous leukemia. During Year 1 of this study, we have precisely mapped the binding site for ubiquitin and generated a p210 BCR/ABL mutant that is completely impaired in binding in mammalian cells. Loss of ubiquitin binding does not destabilize p210 BCR/ABL thus allowing us to directly evaluate the mutant in assays for leukemogenic activity. This mutant will provide us with a unique opportunity to directly assess the importance of the ubiquitin-p210 BCR/ABL interaction in CML. These studies will be performed in Year 2 of this study.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2008
- Accession Number
- ADA488129
Entities
People
- Ian P. Whitehead
Organizations
- University of Medicine and Dentistry of New Jersey