Structural Studies on Toxins and Virulence Factors of Yersinia pestis

Abstract

In this final report we present our efforts on two or three fronts. We were working on the expression purification and crystallization of complexes of YopB and YopD with other proteins like their chaperones. We have made progress in purifying YopB:YopD:SycD complex. However this is a challenging process and the amount of homologous protein is a problem. Since the expression levels of different components of the complex are different the stoichiometric ratio is a problem. We will continue to work on this project to successfully end it even though the project period has ended. If successful this will help in understanding the translocation mechanism of effector proteins.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2008
Accession Number
ADA489666

Entities

People

  • Subramanyan Swaminathan

Organizations

  • Brookhaven National Laboratory

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Aqueous Solutions
  • Biological Toxins
  • Biomedical Research
  • Cell Membrane
  • Cells
  • Crystallization
  • Department Of Defense
  • Diffraction
  • Epithelial Cells
  • Gel Electrophoresis
  • Immune System
  • Molecular Weight
  • Peptides
  • Proteins
  • Three Dimensional
  • Virulence

Readers

  • Molecular and Cellular Biochemistry
  • Systems Analysis and Design
  • Technical Research and Report Writing.