CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer
Abstract
We utilized 97 healthy, 77 benign prostatic hyperplasia (BPH) and 156 prostate cancer (PC) samples that are pre-existing from African-American and Caucasian patients. Due to human subjects issues, the project has been on hold from Dec 2005 till Jan 2008 and thus, results reported are based on a period of approximately two years. In the first hypothesis, we have determined CYP1B1 protein to belocalized to the cytoplasm of PC cells, with levels being much higher in PC than BPH. In the second hypothesis, single nucleotide polymorphisms (SNPs) of CYP1B1 have been evaluated to determine if they are risk factors for race-related PC. Racial differences are observed in allele frequencies as the variant at codons 119 and 432 are greater among Blacks (P<0.001) whereas the 453 variant is predominant in Whites (P<0.001). Within race, a case-control study show the variant at codon 453 plays a protective role for PC among Blacks (P<0.05). Interestingly, SNPs at codons 432 and 449 are determined to be linked and the 432G-449C haplotype was observed to be a risk for PC (P<0.05). In a sampling of cases, no differences were observed between stages (<T2c vs >T2c) and grades (<7 vs >7) of PC in either race. A no-cost extension has been submitted for the 3rd year for which continuation of SNP studies with additional samples to be collected; as well as further experimentation with aim #1 to be performed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2008
- Accession Number
- ADA490383
Entities
People
- Yuichiro Tanaka
Organizations
- Northern California Institute for Research and Education