A Role for MEK-Interacting Protein 1 (MP1) in Hormone Responsiveness of Estrogen Receptor-Positive Breast Cancer Cells

Abstract

The goals of this research are to test the hypothesis that the small scaffold protein MP1 is required for ER function and proliferation of ER- positive breast cancer cells and to characterize the ER/MP1 complex. MP1 expression was inhibited in several breast cancer cell lines by transfection with MP1-targeting siRNAs. The results obtained demonstrate that MP1 is required for the survival of ER-positive MCF-7 cells but not ER-negative MDA-MB-231 cells. This suggests that the requirement for MP1 may be specific to ER-positive cells in which case it could provide a novel target for treatment of this class of breast tumor. To facilitate studying the ER/MP1 complex a Flag-MP1 gene has been cloned and reconstituted into a retrovirus vector. Cells infected with this virus efficiently express the Flag-MP1 gene for at least 9 days and stable cell lines containing this construct are currently being selected for use in characterizing a novel ER/MP1 complex.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2008
Accession Number
ADA491116

Entities

People

  • Susan E. Conrad

Organizations

  • Michigan State University

Tags

DTIC Thesaurus Topics

  • Azo Compounds
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Culture Techniques
  • Knocking
  • Medical Personnel
  • Neoplasms
  • Proteins
  • Survival
  • Targeting
  • Targets
  • Transcription Factors
  • Transfection
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Genetics