Direct Nanoscale Conversion of Biomolecular Signals into Electronic Information
Abstract
This project focused on the design and integration ofbiological sensors with nanoelectronic circuitry, using a diverse array of live redox proteins and biomolecules and based on the development of innovative linkers between the biological systems and the nanodevices. Six different redox proteins (GOx, ADH, GO, LAX, ChOx, Hb) were assembled onto carbon nanotube array electrodes in a site-specific manner by complementary DNA hybridization. The DNA links are renewable and show direct electron transfer. Engineered peptide linkers assembled Npx to nanostructured Au electrodes, providing sensitive detection of peroxide. A membrane-capture fabrication method integrated gold nanopore sensor elements with on-chip micro cyclic voltammetry (demonstrated with GOx). These results encompass (1) a diverse array ofbiomolecules (seven redox active proteins, plus DNA itself); (2) innovative linkers, including addressable links%based on DNA and engineered synthetic biological links; and (3) multiple functional biological-to-nanoelectronic interfaces, including carbon nanotubes, gold nanowires, nanodots, and nanopores.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 22, 2008
- Accession Number
- ADA492481
Entities
People
- Jin Xu
- R. Beresford
Organizations
- Brown University