Metabolic Mapping of Breast Cancer with Multiphoton Spectral and Lifetime Imaging
Abstract
Tumor cells have altered metabolic states compared to their normal cellular counterparts. We found that fluorescence lifetime imaging of breast carcinoma and breast epithelial cells demonstrated changes in the fluorescent lifetime of bound NADH. Interestingly, there were significant metabolic plasticity differences between carcinoma cells and breast cell lines. In breast epithelial cells (MCF10A) there was a significant decrease in the fluorescence lifetime and percent contribution of protein bound NADH as the cell plating density increased. However, in carcinoma cells (T47D and MDA-MB-231) the lifetime and contribution of protein bound NADH was not responsive to cell plating density. Our findings demonstrate that fluorescence lifetime imaging can be used to differentiate normal and malignant cells and may be useful to help characterize tumor onset and progression on the basis of metabolic state. In order to investigate and verify changes in binding proteins, a polarization anisotropy decay measurements system is under development.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2008
- Accession Number
- ADA493646
Entities
People
- Long Yan
Organizations
- University of Wisconsin–Madison