Anti-Inflammatory Cytokine II-10 and Mammary Gland Development

Abstract

The purpose of the award is to compare mammary glands from wildtype and IL-10 knockout mice at critical stages of development. IL10-knockout mice were imported and maintained in University at Buffalo lab animal specific pathogen-free facility. During the first year of the funding (July 06-July 07), we established protocols in our lab for genotyping, mammary gland whole mount preparation and the preparation of paraffin sections of mammary glands. We then mated IL10+/- male with IL10+/- females to generate 144 experimental female mice with +/+ or -/- genotype (72 each) from 80 litters. After excising and processing the glands at 5 critical stages of mammary gland development as described in the grant, we developed data analysis methods to perform the planned comparison. There were no differences between the wildtype (IL10+/+) and IL10-/- mice at up to age day 55. At age day 80 and 150, IL10-/- mice had less terminal end buds suggesting an alteration in mammary gland development. Part of the results are presented in the 2008 Era of Hope meeting (meeting abstract attached).

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2008
Accession Number
ADA493657

Entities

People

  • Shiu-ming Kuo

Organizations

  • University at Buffalo

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Alkanes
  • Biomedical Research
  • Breast Cancer
  • Computer Programs
  • Cytokines
  • Data Analysis
  • Fat Cells
  • Genetics
  • Glands
  • High Resolution
  • Lymph Nodes
  • Lymphatic System
  • Mammary Glands
  • New York
  • Terminals
  • Universities

Readers

  • Aviation Safety and Air Traffic Management
  • Clinical Trial Research.
  • Molecular and genetic basis of cancer.