Understanding the Mechanisms of Platelet Alloimmunization and its Prevention
Abstract
The purpose of our experiments is to identify methods of preventing alloimmunization to donor platelets in a dog platelet transfusion model. We ave established DLA Class II typing to select antigen incompatible donor-recipient pairs for our transfusion experiments. In addition, we are evaluating potential allostimulatory WBC that must be removed to prevent platelet alloimmunization or WBCs that must remain to induce tolerance to donor platelet transfusions. Flow cytometry techniques using antisera that detect various classes of WBCs are being used to identify cells that are removed versus those that remain using different leukoreduction filters. The result of these white cell characterization studies will be correlated with the results of donor platelet transfusion experiments where different filters have been used to leukoreduce donor platelets prior to transfusion. Our next platelet transfusion experiments will evaluate -irradiation alone or combined with leukoreduction as methods of preventing alloimmunization to donor platelets.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2008
- Accession Number
- ADA493973
Entities
People
- Sherrill J. Slichter