Role of STAT5b in Breast Cancer Progression and Metastasis

Abstract

The signal transducer and activator of transcription (STAT) 5b is involved in tumorigenic signaling in a number of cancers including breast cancer. However, its importance in breast cancer migration has not been investigated. This research aims to determine the role of STAT5b in breast cancer migration. Knockdown of STAT5b inhibited migration of human breast cancer cells to serum by 60-80%. This inhibition could be rescued by re-introduction of wild-type STAT5b as well as a transcriptionally inactive Y699F-STAT5b mutant. This suggests that the transcriptional activity of STAT5b is not required for its pro-migratory function. Knockdown of STAT1 also inhibited migration of MDA-MB-231 cells but knockdown of STAT3 or STAT5a had no effect. This data demonstrates that STAT5b is integral to breast cancer cell migration and that this effect may occur through a novel, non-transcriptional function. Further elucidating the mechanism by which STAT5b promotes migration of breast cancer cells may lead to more effective therapies for preventing and treating metastasis.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2008
Accession Number
ADA495317

Entities

People

  • Teresa Bernaciak

Organizations

  • University of Virginia

Tags

DTIC Thesaurus Topics

  • Adhesion
  • Albumins
  • Azo Compounds
  • Biomedical Research
  • Breast Cancer
  • Cancer
  • Cell Line
  • Cell Movement
  • Cell Physiological Processes
  • Cells
  • Department Of Defense
  • Growth Factors
  • Inhibition
  • Metastasis
  • Neoplasms
  • Proteins
  • Transcription Factors

Fields of Study

  • Biology
  • Medicine

Readers

  • Cellular and Molecular Pathways of Apoptosis.
  • Molecular Biology and Genetics
  • Oncology (Cancer Research).