In Vitro Correlate of Immunity in a Rabbit Model of Inhalational Anthrax
Abstract
A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84-133 LD,,) of Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 154 and 1:256) had deaths in their groups. ~esultsh owed that antibody levels to PA at both 6 and 10 weeks were significant (P < 0.0001) predictors of survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2001
- Accession Number
- ADA495566
Entities
People
- A. M. Friedlander
- B. E. Ivins
- J. Barth
- J. Hewetson
- M. Dertzbaugh
- M. L. Pitt
- P. Fellows
- P. Gibbs
- S. F. Little
Organizations
- United States Army Medical Research Institute of Infectious Diseases