Function of Klotho and is MicroRNA in Prostate Cancer and Aging
Abstract
We have observed the expression of CD164, IGFR, and Klotho proteins in human prostate cancer tissue microarrays as determined by immunohistochemistry. A positive correlation between CD164, IGFR and stages of prostate cancer was observed whereas a negative correlation between Klotho and stages was detected. However, the expression of Klotho in terms of the age of patients was not conclusive due to a small number of older (>65 years) patients. Previously, we have identified an intronic microRNA (miRNA) from the Klotho gene; one of the target genes of this miRNA is CD164, therefore, termed mir-CD164. We have conducted in situ hybridization of the mir-CD164 in non-cancer prostate and prostate cancer tissues. Staining was completely absent in prostate cancer samples whereas all of non-cancer tissues investigated showed strong staining for this miRNA, suggesting that human prostate cancer cells frequently have loss of the expression of mir-CD164. We have demonstrated a reverse correlation between the identified intronic miRNA of Klotho gene, mir-CD164, and prostate cancer. Mir-CD164-expressing vectors have been designed and their effect on CD-164 expression in prostate cancer cell lines is in progress.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2008
- Accession Number
- ADA495578
Entities
People
- Shao-yao Ying
Organizations
- University of Southern California