Effects of Acute and Chronic Exercise on Disulfide-Linked Growth Hormone Variants

Abstract

Purpose: To test the hypothesis that the appearance of disulfide-linked growth hormone (GH) aggregates during and after an acute resistance exercise test (ARET) in men could be influenced by chronic physical training. Fourteen men (28 + or - 1 yr) underwent two different 8-wk physical training programs designed to improve military performance. Before and after chronic training, subjects performed an ARET (six sets of 10 repetition-maximum squat) and had venous blood drawn pre-, mid-, and post-ARET (0, 15, and 30 min. post exercise). To determine whether GH molecules were disulfide-linked, serum samples were chemically reduced via glutathione (GSH). Serum immunoreactive GH (IRGH) and immunofunctional GH (IFGH) concentrations were determined using two specific immunoassays, in nonreduced (-GSH) and reduced (+GSH) states. Data were analyzed using repealed-measures ANOVA. Results: No differences were observed in the GH responses of the two training programs; therefore, training group data were combined for analysis.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2009
Accession Number
ADA495780

Entities

People

  • Alexander P. Tuckow
  • Bradley C. Nindl
  • Everett A. Harman
  • Jeffery S Staab
  • Joseph A. Alemany
  • Joseph R. Pierce
  • Kevin R. Rarick

Organizations

  • United States Army Research Institute of Environmental Medicine

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antibodies
  • Assays
  • Body Composition
  • Body Weight
  • Cell Physiological Processes
  • Cells
  • Data Analysis
  • Experimental Design
  • Immunoassay
  • Intensity
  • Measurement
  • Medical Personnel
  • Military Research
  • Molecules
  • Resistance
  • Standards
  • Statistical Analysis

Fields of Study

  • Medicine

Readers

  • Aerospace Propulsion Engineering.
  • Exercise and Sports Science.
  • Molecular and Cellular Biochemistry