Molecular Targets for the Prevention of Prostate Cancer
Abstract
The objectives of this proposal are to determine whether protein kinase C epsilon (PKCe) is linked to the initiation and progression of Prostate cancer (PCa) and should be explored as a molecular target for the prevention of human PCa. PKCe, a calcium-insensitive PKC, is among the PKC isoforms expressed in both mouse and human prostate tissue. We plan to test the hypothesis that PKCe is linked to the onset, progression and metastasis PCa. Two specific aims are proposed to test this hypothesis. Specific Aim #1: To obtain the first molecular genetic evidence that PKCe is linked to the development of PCa. To accomplish this specific aim, we will employ TRAMP mice, the well established mouse model of PCa. We will deplete PKCe in TRAMP mice by crossbreeding TRAMP mice with PKCe knockout (-/-) mice. We will evaluate TRAMP-PKCe KO mice for the development and progression of PCa in vivo. We will determine whether the genetic loss of one (-/+) or both (-/-) PKCe alleles will attenuate the progression of PCa. Specific Aim #2: To explore the mechanisms by which PKCe may promote the progression of AI PCa. This report will review the accomplishments made over the first year of grant award with respect to these specific objectives and according to the time line proposed in the original statement of work of the project.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2008
- Accession Number
- ADA497253
Entities
People
- Ajit K. Verma
Organizations
- University of Wisconsin–Madison