Proteomic Analysis of Prostate Cancer Field Effect
Abstract
We have undertaken a proteomic approach to identify proteins altered in both cancer and benign cells near the cancers. This study takes advantage of the ?field effect? of the cancer, the malignancy associated changes in normal cells to discover cancer altered protein that could be more abundant in serum or urine since they would come from both benign and cancer cells. We have set optimal conditions for laser capture microdissection (LCM) of prostate epithelia and extraction of protein from them for the mass spectroscopic (MS) analysis of resultant peptides and protein identification through available soft ware programs. We changed the control approach to our study since cystoprostatectomy prostate tissues did not become available, but we were given cross sections of prostates. Whereas we originally had planned to use a pool of cystoprostatectomy prostate epithelia as a control in each iTRAQ run, we now are able to have controls internal to the individual prostate specimen; i.e., benign distant to cancer and benign prostatic hyperplastic (BPH) tissues vs cancer and benign near cancer tissues. In addition, use of new state of the art equipment in the Mass Spectroscopy and Proteomics Core Facility of the University of Minnesota and implementation of the 8iTRAQ labeling method will increase the data acquisition capability of our studies.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 2008
- Accession Number
- ADA497256
Entities
People
- Michael J. Wilson
Organizations
- University of Minnesota