Proteomic Analyses of Nipple Fluid for Early Detection of Breast Cancer
Abstract
Our purpose is to combine the well-established method of breast nipple fluid collection with new proteomics methodology for analyses of complex protein mixtures, in order to seek a better test for early breast cancer. The scope of our work is far-reaching, as our results could have a significant impact on the ability to detect occult breast cancers at earlier stages than is possible with current cancer detection methods. To date, we have completed a pilot test of 12 patients to optimize collection and lab storage and analysis conditions. Throughout the last year, we have completed sample collection: we have collected a total of 255 nipple aspirate fluid (NAF) samples (58 case samples and 197 control samples). Our modification of the collection protocol to collect NAF directly with capillary has been successful. This collection procedure is more efficient providing more useable samples for lab analysis. We have run test samples using SELDI-TOF methodology that showed significant numbers of peaks on IMAC40 chips thereby validating both the collection and analysis protocols. In summary, we have developed a workable protocol for nipple fluid collection that produces consistent quantitative protein analysis. We have completed sample collection and will turn our attention to sample analysis in the coming year.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 14, 2008
- Accession Number
- ADA497821
Entities
People
- Judy Garber
Organizations
- Dana–Farber Cancer Institute