Quantifying Biomarkers of Liver Damage Using Shotgun Proteomics
Abstract
In a preliminary examination of labeless protein quantitation proteomics, we investigated the serum composition of rats treated with the hepatotoxin alpha-naphthylisothiocyanate (ANIT) to find biomarkers of exposure. ANIT was administered orally to the rats in doses of 1, 20, and 100 mg/kg. Collected serum was immunodepleted and analyzed using an LTQ mass spectrometer. MS/MS spectra were searched against the rat IPI protein database to identify peptides, and valid peptides were grouped according to protein and quantified using Bioworks v3.2. In examining the dataset, only the proteins with the highest abundance relative to the control population were considered. T-kininogen and other kininogen family proteins were found to increase on average of 3-5 fold in all three treatment groups compared with the control. Complement C9 protein averaged 3- to 6-fold higher versus control. Since ANIT is an accepted model of intrahepatic cholestasis, the data from this study may help indicate cholestasis status.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2006
- Accession Number
- ADA498948
Entities
People
- Camilla A. Mauzy
- Claude C. Grigsby
- Deirdre A. Mahle
- Frank Witzmann
- John J. Schlager
- Lee W. Ott
Organizations
- Indiana University Bloomington