Integrated Semiconductor-based Diagnostics System for Multiplexed Genomic Amplification and Electrochemical Detection of Biothreat Agents
Abstract
This contract effort focused on the development of an automated, cartridge-based genotyping system that integrates a multiplex polymerase chain reaction (PCR) assay with a semiconductor microarray that uses electrochemical detection to identify biothreat agents. The DX-100 Genotyping Cartridge System has three subcomponents: a disposable cartridge, the cartridge processing instrument, and a personal computer. The alpha prototype is compact and solid state (no optics) and has a simple three step operation: 1. Load the cartridge with a DNA sample; 2. Load the cartridge in the instrument; 3. Move the operating handle to the front. A personal computer with integrated software controls the instrument's operation, collects electrical readings from each electrode on the microarray, analyses the data, and provides a graphic output for agent identification. The fluidic cartridge is sealed and contains all of the reagents and a microarray to test for Bacillus anthracis, Clostridium botulinum, Yersinia pestis, Francisella tularensis, Burkholderia pseudomallei, Brucella suis, Vibrio cholera, and Yersinia enterocolitica.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2009
- Accession Number
- ADA499711
Entities
People
- David L. Danley
- Kia Peyvan