Assessment of GPR30, a Seven Transmembrane-Spanning Estrogen Receptor, as an Oncogene
Abstract
Expression of the seven transmembrane-spanning receptor (7TMR), GPR30, in primary human breast tumors is positively associated with several tumor progression variables including extra mammary metastases (Filardo et al, 2006). Altered expression of 7TMRs is linked with a spectrum of disease phenotypes, including cancer, raising the possibility that GPR30 may function as an oncogene. To test this hypothesis, two lines of transgenic mice (T6-1A and T6-2E) were engineered with stably integrated hemagglutinin-tagged (HA-GPR30) transgenes under the transcriptional control of the mouse mammary tumor virus (MMTV) promoter. Endogenous GPR30 protein was readily detected in mammary glands harvested from control and transgenic mice using peptide antibodies. However, breast tissue from nulliparous progeny mice expressed no detectable GPR30 transgene protein or mRNA. MMTV-HA-GPR30 mice exhibited normal reproductive behavior, lactational competence and no overt signs of cancer or premaligancy. Increased transgene expression or abnormal mammary gland growth was also not evidenced in multiparous mice. A second no cost extension was requested to construct mice with an HA-GPR30 transgene regulated by the whey acidic protein(WAP) promoter.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2008
- Accession Number
- ADA499827
Entities
People
- Edward J. Filardo
Organizations
- Rhode Island Hospital