Role of Superoxide Dismutase in the Photochemical Response of Cultured RPE Cells to Laser Exposure at 413 nm

Abstract

Thresholds for photochemical damage were performed in RPE cell lines (artificially pigmented) taken from either human (hTERT-RPE1), wild type (wt) mouse, or transgenic mice deficient (+/-) in either superoxide dismutase 1 (SOD1) or SOD2. The four cell lines were characterized by immunohistochemical and immunoblot analyses to determine relative abundance of the SOD proteins. There was no difference in sensitivity between the human, murine wt and murine SOD1-deficient cells, whereas there was a dramatic (2 fold) increase in threshold irradiance value for the murine SOD2-deficient cells. Possible explanations for the unexpected result are provided.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2007
Accession Number
ADA500407

Entities

People

  • Kurt J. Schuster
  • Larry E. Estlack
  • Michael L. Denton
  • Michael S. Foltz
  • Robert J. Thomas

Tags

Communities of Interest

  • Energy and Power Technologies
  • Engineered Resilient Systems

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Antibodies
  • Cell Line
  • Cells
  • Central Nervous System
  • Charge Coupled Devices
  • Confocal Microscopy
  • Culture Techniques
  • Cytoplasmic Granules
  • Damage
  • Laser Damage
  • Laser Safety
  • Lasers
  • Military Research
  • Superoxides
  • Waveplates

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Vision Science/Vision Psychology/Cognitive Neuroscience.

Technology Areas

  • Directed Energy