Select Agent Recovery and Identification Using Aptamer-Linked Immoblized Sorbent Assay

Abstract

Environmental recovery and identification technology for Select Agents (potential biological warfare or terrorism agents) requires the capability of a flexible and rapid response, whose rapidity and flexibility exceed the current capability of immunodiagnostic assays. These new assays also need to be brought as far forward in the "Area of Responsibility" as possible to facilitate the appropriate sequestration of exposed areas, handling of exposed individuals and expected casualties. Therefore, any such new assays must be extremely robust and require a minimal logistic tail. We present here examples of such a new technology, Aptamer-Linked Immobilized Sorbent Assays (ALISA). A form of this aptamer-linked assay was used recently in Houston, Texas, to address a tularemia alarm of Biowatch. It compared favorably to a number of standard microbiological techniques and immunoassays. We present here formats for this assay that go further to meet the robustness requirement than the standard ELISA-like format.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2008
Accession Number
ADA500447

Entities

People

  • Eric A. Holwitt
  • Johnathan L. Kiel
  • Veronica K. Sorola

Organizations

  • Air Force Research Laboratory

Tags

DTIC Thesaurus Topics

  • Air Force
  • Air Force Research Laboratories
  • Anhydrides
  • Biological Warfare
  • Casualties
  • Chemical Synthesis
  • Chemistry
  • Identification
  • Materials Laboratories
  • Nanocrystals
  • Organic Chemistry
  • Particles
  • Quantum Dots
  • Recovery
  • Standards
  • Therapy
  • Warfare

Readers

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