Molecular Modulation of Inhibitors of Apoptosis as a Novel Approach for Radiosensitization of Human Prostate Cancer

Abstract

The major goal of the project is to investigate the radiosensitization activity and mechanism of action of novel IAP-inhibitors in prostate cancer. In the third year of the project, we have investigated the in vivo radiosensitization activity of our lead IAPinhibitors, SH-130 and Embelin, and mechanism of action in human prostate cancer xenograft model. IAP-inhibitors potently enhanced radiation-induced tumor growth inhibition, together with increased induction of apoptosis. In nude mouse xenograft models, IAP-inhibitors Embelin and SH-130 potently sensitized the DU-145 tumors to X-ray radiation. Mechanism studies show that NK-kB pathway activation was also inhibited in the combination therapy. Interestingly, we also observed anti-angiogenic effect of the combination treatment. Due to the lab move in March, 2008, together with our animal room moved and more importantly the move of our X-ray irradiator at the same time, our animal experiments were delayed. We have requested and obtained approval a one year no-cost extension for finish the planned animal and MOA studies.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2008
Accession Number
ADA501638

Entities

People

  • Liang Xu

Organizations

  • University of Michigan

Tags

DTIC Thesaurus Topics

  • Antineoplastic Agents
  • Body Weight
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chemotherapy
  • Combination Therapy
  • Epithelial Cells
  • Gene Expression
  • Ionizing Radiation
  • Neoplasms
  • Programmed Cell Death
  • Proteins
  • Three Dimensional
  • X Rays

Fields of Study

  • Biology
  • Medicine

Readers

  • Clinical Trial Research.
  • Oncology (Cancer Research).
  • Parasitology and Pharmacology of Malaria.