Isolation and Growth of Prostate Stem Cells and Establishing Cancer Cell Lines from Human Prostate Tumors
Abstract
The objective of this proposal was to isolate, grow, and characterize normal prostate stem cells and establish new prostate cancer cell lines from fresh human prostate tissues. The hypothesis is that prostate stem cells express defined stem cell markers, self-renew, and require the use of a feeder layer which is necessary for the establishment of prostate cancer cell lines from primary tumors. The goal of Specific Aim I was to test the hypothesis that normal human prostate stem cells express markers of other tissue stem cells, require a defined in vitro growth environment for self-renewal and differentiation, generate progeny that differentiate into cells found within the prostate epithelial compartment, and form functioning prostatic glandular structures in vivo. We have completed all the work set forth in Specific Aim I and have submitted the manuscript encompassing this aim for peer review. In addition, we have conducted an in depth analysis of stem cell marker expression in prostate cancer cell lines and the concomitant cancer initiating cell ability.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2008
- Accession Number
- ADA501832
Entities
People
- Donald J Vander Griend
Organizations
- Johns Hopkins Hospital