A Myc-Driven in Vivo Model of Human Prostate Cancer
Abstract
The long-term goal of the work proposed here is to generate, characterize and interrogate human epithelial cell-based in vivo models of prostatic carcinogenesis. These models will allow an examination of processes involved in carcinogenesis, tumor growth and metastasis. Since the tumors are themselves of human origin they represent an in vivo test bed to examine both tumor biology and the application of therapeutic agents. Over the lifetime of this grant we have developed a new metastatic model based upon overexpression of c-Myc in primary cultures of human prostatic epithelial cells. This model was extremely aggressive and we therefore altered the insult from high overexpression of c-Myc towards a more moderate suppression of PTEN. This resulted in a premalignant phenotype which we continue to explore. Concurrently we have developed two new human prostatic epithelial cell lines which, in tissue recombinants behave like normal prostatic epithelia but are able to act as recipients for virally transduced genetic insults providing a basis for new cancer models and a resource for the community. These have been distributed and a descriptive paper submitted. We also developed a new orthotopic model of prostate cancer metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2008
- Accession Number
- ADA502009
Entities
People
- Simon V. Hayward
Organizations
- Vanderbilt University Medical Center