Identification of Chromosome 18q Transcripts Lost in Breast Cancer
Abstract
A novel homozygous region of loss at 18q22.3 was detected in 50% of breast tumors by array comparative genomic hybridization. There are no known genes located within this region. Since chromosomal regions exhibiting homozygous deletion are not commonly found and are usually the site of tumor suppressor genes, the following hypothesis was proposed: Encoded within the region of homozygous deletion at 18q22.3 is a transcript whose loss plays a role in the development or progression of breast cancer. A custom oligonucleotide expression microarray covering the deleted region was designed and synthesized. RNA was isolated from primary human mammary epithelial cells (HMEC; Lonza), converted to fluorescently-labeled complementary RNA and hybridized to the microarray. An approximately 500 base pairs sequence was found to be transcribed in normal breast epithelial cells. This is a novel transcript that has not been described previously. We confirmed the existence of this transcript with a quantitative PCR assay using normal breast epithelial cell cDNA and are analyzing the level of this transcript in RNA isolated from commercially-available breast cancer cell lines.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2008
- Accession Number
- ADA502499
Entities
People
- Teresa L Johnson-Pais
Organizations
- University of Texas Health Science Center at San Antonio