A Metagenomic Analysis of Microbial Contamination in Aviation Fuels

Abstract

Microbial contamination in aviation fuel arises due to the impracticality of keeping fuel tanks sterile and the inevitable presence of water from condensation. Microbial contaminants in aviation fuels are a concern because of their potential to degrade the fuel, accelerate corrosion within the fuel tank, and threaten flight safety. This research aids in mitigating those problems by comprehensively characterizing the microbial communities affecting aviation fuels. Advances in molecular biological techniques have allowed for the identification of microorganisms which were not identified by the traditional culture-based methodologies used in previous studies. This study employed a molecular method known as 16S rDNA gene analysis to describe the microbial communities in aviation fuel. The microbial communities in JP-8, Jet A, and biodiesel were evaluated at the phylum and genus levels of taxonomy. The JP-8 community was found to be much richer than both the Jet A and biodiesel community. The biodiesel community was found to be a subset of the JP-8 community. A small subset of microorganisms was found to exist across all three fuels while the majority of identified microorganisms were endemic to a single fuel type. Rarefaction analysis showed that further sampling is likely to reveal additional diversity.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2009
Accession Number
ADA502519

Entities

People

  • Jerrod P. Mccomb

Organizations

  • Air Force Institute of Technology

Tags

Communities of Interest

  • Air Platforms
  • Biomedical
  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteriology
  • Biodegradation
  • Chemical Synthesis
  • Chemistry
  • Fungi
  • Hydrocarbon Fuels
  • Material Degradation Processes
  • Materials Science
  • Materials Testing
  • Microbial Genetics
  • Microbial Genome
  • Microbiology
  • Microbiomes
  • Microorganisms

Fields of Study

  • Biology
  • Environmental science

Readers

  • Microbial Pathology
  • Petroleum Engineering

Technology Areas

  • Biotechnology