Single-Walled Carbon Nanotubes Targeted to the Tumor Vasculature for Breast Cancer Treatment
Abstract
Annexin V has been produced in good purity and high yield has been shown to bind strongly to plastic-immobilized phosphatidylserine (PS). Annexin V was conjugated with a suspension of single-walled carbon nanotubes (SWNTs) and carboxymethyl- cellulose (CMC) that had been dialyzed; this conjugation involves reaction of carboxyl groups of CMC with amino groups of the protein. A binding assay of this complex to human endothelial cells with PS exposed on the cell surface showed strong binding, indicating that the covalently bound annexin V is functional. Tests of laser treatment of endothelial cells on 24-well plates were carried out to determine the energy density range that would allow the cells to grow (less than or equal to 360 J/cm2). However, laser tests of endothelial cells on plates with the SWNT-CMC-annexin V complex present up to an energy density of about 200 J/cm2 did not show a conclusive cell-killing effect. It has subsequently been found that free CMC is not removed to a significant degree by dialysis, indicating that annexin V was probably conjugated mainly to free CMC. Therefore, two alternative methods to attach annexin V to a molecule adsorbed to SWNTs are currently being pursued.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2008
- Accession Number
- ADA502569
Entities
People
- Roger Harrison
Organizations
- University of Oklahoma