The Role of YY1 in Prostate Cancer
Abstract
We conducted renalgraft experiments to study the role of YY1 in prostate cancer. We used the mouse prostate epithelial cells (MPECs) with either depleted endogenous YY1 or overexpressed exogenous YY1. The grafts of YY1-depleted MPECs did not show significant growth. We reasoned this to the culturing of urogenital mesenchyme (UGM) and this study experiment will be repeated. In YY1 increase study, we successfully generate renalgrafts, but did not observe any significant change in the histology and weight in response to YY1 increase. One possible reason is that YY1 does not play an etiological role in prostate tumorigenesis. Another possible reason is that the CMV promoter used to drive YY1 expression was silenced in vivo. We are currently testing chicken beta-actin promoter and an inducible system to overexpress YY1 and repeat the experiments. We have determined the effects of different YY1 levels on the proliferation of MPECs in a 3-D culture system. This will provide insight on choosing an appropriate YY1 expression level in animal studies. We further defined the binding region of Mdm2 on YY1 from previously determined 95 residues to currently 26 residues. This will help us in the structural/functional studies to be carried out in next step.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2009
- Accession Number
- ADA502756
Entities
People
- Guangchao Sui
- Scott D. Cramer
Organizations
- Wake Forest University