Developing a Zebrafish Model of NF1 for Structure-Function Analysis and Identification of Modifier Genes
Abstract
This progress report summarizes the second year of activity of this project focused on the identification and characterization of the zebrafish orthologs of the neurofibromatosis type 1 genes. This project involves work within the Epstein laboratory and collaboration with the laboratory of Dr. Thomas Look at the Dana Farber Cancer Institute as a sub-contract. This progress report summarized the collaborative work including results from both groups. During the first year, significant progress had been made in the isolation and characterization of two zebrafish orthologs, nf1a and nf1b. The genes have been sequenced and their expression patterns identified. The knockdown phenotypes have been characterized and include overgrowth of glia and cardiovascular abnormalities. During the second year, these knockdown phenotypes have been further characterized and stable fish lines harboring mutations in these genes have been generated and procured with additional work underway. Manuscripts have been submitted for publication and are presently being updated to address reviewers? comments.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2009
- Accession Number
- ADA502775
Entities
People
- Jonathan A. Epstein
Organizations
- University of Pennsylvania