Scavenger Receptors and Resistance to Inhaled Allergens
Abstract
In vitro we tested our hypothesis that DCs from SRA KO mice would show altered migration and maturation, which would possibly explain the observation of enhanced allergy in the SRA KO mice. In detailed studies of random migration, chemotaxis to two different chemoattractants and surface marker maturation in response to two different stimuli (TNF and LPS) we found no difference in SRA deficient vs wild type dendritic cells. We made significant progress in establishing protocols for adoptive transfer studies that will allow testing of SRA deficient dendritic cells in vivo. It remains possible that the in vitro assays do not replicate the actual in vivo environment in which these differences are manifest. Hence, the focus of the remainder of the project will be to test these postulates using in vivo models. Both systemic and intratracheal adoptive transfer experiments will be used to fully test the role of the SRAs in modulating asthma susceptibility and severity in the mouse model. In addition, we made substantial progress in establishing inflammatory responses in SRA knockout mice in responses to oxidant air pollutants, which will allow direct testing of how air pollutants may modify SRA function in allergic responses in the coming period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 2009
- Accession Number
- ADA503160
Entities
People
- Lester Kobzik
Organizations
- Harvard T.H. Chan School of Public Health