Contact Versus Noncontact Cell Electroporation

Abstract

In this research, experiments and simulations were performed to determine the effects of capacitive VS conductive coupling for the electroporation of cells. The experiment is performed with a fast risetime pulser to expose samples of cells to electric fields at peak voltage of 24.4 kV, 0.6 ns risetime, and 1.6 ns FWHM. Experiments performed compare the direct-conductive connection of the cell suspension versus a capacitively coupled cell suspension. The main conclusion of this research is that, for the parameters of this experiment, electroporation does not occur for capacitive coupling (Displacement Current): Conduction current is required for electroporation to occur.

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Document Details

Document Type
Technical Report
Publication Date
May 28, 2009
Accession Number
ADA503363

Entities

People

  • David M. French
  • Michael Uhler
  • R. M. Gilgenbach
  • Y. Y. Lau

Organizations

  • University of Michigan

Tags

Communities of Interest

  • Advanced Electronics

DTIC Thesaurus Topics

  • Air Pressure
  • Capacitance
  • Capacitors
  • Cell Physiological Processes
  • Cells
  • Circuits
  • Couplings
  • Culture Media
  • Displacement
  • Electric Fields
  • Electromagnetic Fields
  • Impedance
  • Lymphocytes
  • Resistance
  • Scaling Laws
  • Simulations
  • Transmission Lines

Readers

  • Electrical Engineering
  • Munitions and Ordnance Engineering